Monoclonal Antibodies to an Ectopically Expressed Alkaline Phosphatase in a Human Mal ignant Cell Line I

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چکیده

The D 9 8 / A H 2 cell line (a subline of HeLa) expresses a form of alkaline phosphatase (ALP) which closely resembles the adult and fetal intestinal forms of ALP. To character ize this ectopic form of ALP, four monocIonal antibodies were raised against D 9 8 / A H 2 ALP, and their binding with ALPs from D 9 8 / AH-2 cells, placenta, fetal intestine (meconium), adult intestine, and liver was compared using an electrophoret ic titration procedure. The ALPs were either untreated or treated with neuraminidase. All four monoclonal antibodies bound desialated D98 /AH-2 ALP most strongly. Adult intestinal ALP, which does not contain sialic acid residues, reacted much more strongly than either sialated or desialated fetal intestinal ALP. Two of the four monoclonal antibodies reacted very weakly or not at all with placental ALP, but two others reacted more strongly with placental ALP than with fetal intestinal ALP. None of the antibodies reacted with liver ALP. From these results, it appears that D 9 8 / A H 2 ALP may be a modified form of adult intestinal ALP.

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Monoclonal Antibodies to an Ectopically Expressed Alkaline Phosphatase in a Human Mal ignant Cell Line I

The D 9 8 / A H 2 cell line (a subline of HeLa) expresses a form of alkaline phosphatase (ALP) which closely resembles the adult and fetal intestinal forms of ALP. To character ize this ectopic form of ALP, four monocIonal antibodies were raised against D 9 8 / A H 2 ALP, and their binding with ALPs from D 9 8 / AH-2 cells, placenta, fetal intestine (meconium), adult intestine, and liver was co...

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تاریخ انتشار 2007